Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17122] to DNAJB6 - N-terminal
  • Suitable for: IP, WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DNAJB6 antibody [EPR17122] - N-terminal
    See all DNAJB6 primary antibodies

  • Description

    Rabbit monoclonal [EPR17122] to DNAJB6 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U87-MG, SH-SY5Y, HeLa and Jurkat lysate. Mouse heart, rat brain, rat heart, C6, RAW264.7 and PC-12 lysate. Human testis tissue. Rat cerebral cortex tissue. SH-SY5Y and Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat heart lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW264.7 cell lysate
    Lane 6 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

    Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

  • Flow Cytometry - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Flow Cytometry - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

    Flow cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

  • Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

    DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
    Lane 2: ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)
    Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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