Anti-DDX18 antibody (ab70527)
Key features and details
- Rabbit polyclonal to DDX18
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX18 antibody
See all DDX18 primary antibodies -
Description
Rabbit polyclonal to DDX18 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A region between residue 625 and the C terminus (residue 670) of human DDX18 (NP_006764.3)
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Positive control
- Whole cell lysate from HeLa and 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX18 antibody (ab70527)All lanes : Anti-DDX18 antibody (ab70527) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DDX18 antibody (ab70527)Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70527 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
Chemiluminescence with an exposure time of 30 seconds.
