Anti-CoCoA antibody (ab70564)
Key features and details
- Rabbit polyclonal to CoCoA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CoCoA antibody
See all CoCoA primary antibodies -
Description
Rabbit polyclonal to CoCoA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 650 and the C terminus (residue 691) of human CoCoA (NP_065949.1)
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Positive control
- Whole cell lysate from: HeLa, 293T, NIH3T3, Ramos and MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70564 was affinity purified using an epitope specific to CoCoA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CoCoA antibody (ab70564)All lanes : Anti-CoCoA antibody (ab70564) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lane 4 : Ramos whole cell lysate
Lane 5 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-CoCoA antibody (ab70564)Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
Lane 3: Control IgG immunoprecipitate.
Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
Detection was by chemiluminescence with an exposure time of 30 seconds.
