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Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)

Price and availability

526 012 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15375(B)] to DCI - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-DCI antibody [EPR15375(B)] - BSA and Azide free
    See all DCI primary antibodies
  • Description

    Rabbit monoclonal [EPR15375(B)] to DCI - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250903 is the carrier-free version of ab186752. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250903 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15375(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Images

  • Western blot - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Western blot - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/50000 dilution

    Lane 1 : Human fetal liver cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa



    This data was developed using ab186752, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Immunocytochemistry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)

    This data was developed using ab186752, the same antibody clone in a different buffer formulation.

    Immunofluorescence analysis of HeLa cells labeling DCI with ab186752 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
  • Flow Cytometry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Flow Cytometry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    This data was developed using ab186752, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling DCI with ab186752 at 1/240 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Immunocytochemistry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Immunocytochemistry - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    This data was developed using ab186752, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7cells labeling DCI with ab186752 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    This data was developed using ab186752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    This data was developed using ab186752, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Western blot - Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/10000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Lanes 3-4 : Goat Anit-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa



    This data was developed using ab186752, the same antibody clone in a different buffer formulation.

     

  • Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)
    Anti-DCI antibody [EPR15375(B)] - BSA and Azide free (ab250903)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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