Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)
![Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)](https://www.abcam.com/ps/products/181/ab181832/Images/ab181832-410564-anti-cytokeratin-7-antibody-ep1620y-bsa-and-azide-free-western-blot-mouse.jpg "Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1620Y] to Cytokeratin 7 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free
See all Cytokeratin 7 primary antibodies -
Description
Rabbit monoclonal [EP1620Y] to Cytokeratin 7 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab181832 is the carrier-free version of ab68460 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab181832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1620Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)All lanes : Goat Anti-Rat IgG H&L (FITC) (ab6840) at 1/10000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : KRT7 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab68460, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab68460 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68460 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab68460 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)](https://www.abcam.com/ps/products/181/ab181832/Images/ab181832-3-anti-cytokeratin-7-antibody-ep1620y-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)This data was developed using ab68460, the same antibody clone in a different buffer formulation.ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab68460 & T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)](https://www.abcam.com/ps/products/181/ab181832/Images/ab181832-405508-anti-cytokeratin-7-antibody-ep1620y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg/ml
Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg/ml
Lane 4 : Human placenta tissue lysate at 20 mg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDaThis data was developed using ab68460, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
~45 kDa bands are supported by the literature and competitor's products, which may be an isoform (PMID: 16265353).
-
![Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)](https://www.abcam.com/ps/products/181/ab181832/Images/ab181832-402312-anti-cytokeratin-7-antibody-ep1620y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaThis data was developed using ab68460, the same antibody clone in a different buffer formulation.
-
![Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)](https://www.abcam.com/ps/products/181/ab181832/Images/ab181832-5-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 7 antibody [EP1620Y] - BSA and Azide free (ab181832)