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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [KRT20/1993] to Cytokeratin 20
  • Suitable for: WB, IHC-P, Protein Array
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Cytokeratin 20 antibody [KRT20/1993]
    See all Cytokeratin 20 primary antibodies
  • Description

    Mouse monoclonal [KRT20/1993] to Cytokeratin 20
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Cytokeratin 20 aa 196-323. The exact sequence is proprietary.
    Database link: P35900

  • Positive control

    • WB: HT29 cell lysate. IHC-P: Human colon and colon carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    KRT20/1993
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

    Formalin-fixed, paraffin-embedded human normal colon tissue stained for Cytokeratin 20 using ab268107 at 2 μg/ml in immunohistochemical analysis. 

  • Western blot - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
    Western blot - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
    Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107) at 2 µg/ml + HT29 (Human colorectal adenocarcinoma cell line) cell lysate

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab268107 at 2 μg/ml in immunohistochemical analysis. 

  • Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)
    Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1993] (ab268107)

    Protein Array containing more than 19,000 full-length human proteins using ab268107.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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