Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8837] to CXCR5 - Low endotoxin, Azide free
  • Suitable for: ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free
    See all CXCR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR8837] to CXCR5 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: Raji and Ramos cells.

  • General notes

    ab225575 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)
    Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)

    Confocal image showing cytoplasmic staining on Raji (human Burkitt's lymphoma) cells. 

    Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling CXCR5 with unpurified ab133706 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    -ve control 1: ab133706 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133706).

  • OI-RD Scanning - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)
    OI-RD Scanning - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133706).

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)
    Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)

    This ICC/IF data was generated using the same anti-CXCR5 antibody clone [EPR8837] in a different buffer formulation (cat# ab133706).

    Confocal image showing cytoplasmic staining on Ramos (human Burkitt's lymphoma) cells. 

    Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling CXCR5 with ab133706 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    -ve control 1: ab133706 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

  • Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)
    Anti-CXCR5 antibody [EPR8837] - Low endotoxin, Azide free (ab225575)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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