Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
![Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851-2-ab75851FC.jpg "Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cullin 3/CUL-3 antibody [EPR3196Y]
See all Cullin 3/CUL-3 primary antibodies -
Description
Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human Cullin 3/CUL-3 aa 750-850 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3196Y -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851wb.jpg)
Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)All lanes : Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Observed band size: 82,90 kDa why is the actual band size different from the predicted?
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![Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.com/ps/products/75/ab75851/Images/ab75851-3-benefits-of-recombinant-antibodies.png)
Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
