Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CT A antibody [EPR3940(2)] (ab109263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3940(2)] to CT A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CT A antibody [EPR3940(2)]
    See all CT A primary antibodies

  • Description

    Rabbit monoclonal [EPR3940(2)] to CT A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A549, K562 , Mouse brain, and RAW 264.7 lysates. IHC-P: human colon, human prostatic hyperplasia tissue and mouse pancreas tissues. ICC/IF: HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Previously labelled as PCYT1A. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling CT A with Purified ab109263 at 1:1000 dilution (0.53 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CT A with Purified ab109263 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CT A with Purified ab109263 at 1:500 dilution (1.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified) + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
    All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

    ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

    ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CT A antibody [EPR3940(2)] (ab109263)
    Anti-CT A antibody [EPR3940(2)] (ab109263)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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