Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)
![Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)](https://www.abcam.com/ps/products/247/ab247452/Images/ab247452-397951-anti-crk-p38-phospho-y221-antibody-epr269y-bsa-and-azide-free-western-blot.JPG "Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR269Y] to Crk p38 (phospho Y221) - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free
See all Crk p38 primary antibodies -
Description
Rabbit monoclonal [EPR269Y] to Crk p38 (phospho Y221) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects proteins phosphorylated at tyrosine 221.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247452 is the carrier-free version of ab76227 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247452 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR269Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaThis data was developed using ab76227, the same antibody clone in a different buffer formulation.
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![Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)](https://www.abcam.com/ps/products/247/ab247452/Images/ab247452-2-benefits-of-recombinant-antibodies.png)
Anti-Crk p38 (phospho Y221) antibody [EPR269Y] - BSA and Azide free (ab247452)
