Anti-CREST antibody (ab227535)
Key features and details
- Rabbit polyclonal to CREST
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CREST antibody
See all CREST primary antibodies -
Description
Rabbit polyclonal to CREST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human CREST (internal sequence). The exact sequence is proprietary.
Database link: O75177 -
Positive control
- WB: Mouse brain lysate; HeLa whole cell and nuclear lysates. IP: MCF7 whole cell extract. IHC-P: Huh-7 xenograft tissue. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-CREST antibody (ab227535) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 43 kDa10% SDS-PAGE gel.
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All lanes : Anti-CREST antibody (ab227535) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa nuclear lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa10% SDS-PAGE gel.
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Paraffin-embedded Huh-7 xenograft tissue stained for CREST with ab227535 at 1/500 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CREST (green) using ab227535 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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CREST was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 μg ab227535. Western blot was performed from the immunoprecipitate using ab227535.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab227535 IP in MCF7 whole cell extract.