Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes

Anti-CREST antibody (ab227535)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-CREST antibody (ab227535)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CREST
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Product image
Human FUS (TLS) knockout HEK293T cell pellet (ab278884)
Product image
Anti-Elf4/MEF antibody (ab96075)
Product image
Anti-AF4 antibody (ab262920)
Product image
Anti-Tcl1 antibody [SP248] - BSA and Azide free (ab242426)

Overview

  • Product name

    Anti-CREST antibody
    See all CREST primary antibodies
  • Description

    Rabbit polyclonal to CREST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human CREST (internal sequence). The exact sequence is proprietary.
    Database link: O75177

  • Positive control

    • WB: Mouse brain lysate; HeLa whole cell and nuclear lysates. IP: MCF7 whole cell extract. IHC-P: Huh-7 xenograft tissue. ICC/IF: A431 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Western blot - Anti-CREST antibody (ab227535)
    Western blot - Anti-CREST antibody (ab227535)
    Anti-CREST antibody (ab227535) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-CREST antibody (ab227535)
    Western blot - Anti-CREST antibody (ab227535)
    All lanes : Anti-CREST antibody (ab227535) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa nuclear lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREST antibody (ab227535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREST antibody (ab227535)

    Paraffin-embedded Huh-7 xenograft tissue stained for CREST with ab227535 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (ab227535)
    Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (ab227535)

    Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CREST (green) using ab227535 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Immunoprecipitation - Anti-CREST antibody (ab227535)
    Immunoprecipitation - Anti-CREST antibody (ab227535)

    CREST was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 μg ab227535. Western blot was performed from the immunoprecipitate using ab227535.

    Lane 1: Control IgG IP in MCF7 whole cell extract.

    Lane 2: ab227535 IP in MCF7 whole cell extract.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CREST antibody (ab227535)

  •  
  • Product image

    Anti-CREST antibody (ab126126)

    Applications: WB

  •  
  • Product image

    Anti-CREST antibody (ab222312)

    Applications: ICC/IF

  •  
  • Product image

    Anti-CREST antibody (ab222835)

    Applications: IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-KPNA3 antibody (ab84706)

  •  
  • Product image

    Anti-ARPC2 antibody (ab96779)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.