Anti-CREST antibody (ab222835)
Images
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Paraffin-embedded human gastric cancer tissue stained for CREST using ab222835 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for CREST using ab222835 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CREST antibody (ab222835) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse spleen tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 30,34,41,43,44 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?