Catalase beads (ab218718)
Key features and details
- Sample type: Cell Lysate, Plasma, Serum, Urine
Overview
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Product name
Catalase beads -
Sample type
Urine, Serum, Plasma, Cell Lysate -
Product overview
Catalase beads (ab218718) are used to remove hydrogen peroxide from biological samples prior to downstream analyses where the presence of hydrogen peroxide causes interference. They can be used on urine, serum, plasma or cellular extracts. ab218718 is well-suited for high throughput preparation protocols.
ab218718 is manufactured using a proprietary method to covalently attach catalase to 6% cross-linked agarose beads. The coupling technique is optimized to give a high yield and recovery of catalase activity, and to ensure long term stability of the immobilized enzyme.
ab218718 is available in 1 or 10 mL sizes of a 50% slurry of catalase agarose beads in PBS, with a capacity of 5 or 50 mL of sample, respectively.
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Notes
Protocol
You will also require 96-well polypropylene plates and a centrifuge for 96-well plates.
- Mix catalase beads gently to generate a homogeneous suspension (do not invert the tube).
- Add 10 μL of the catalase bead suspension to 50 μL of your sample.
- Incubate the sample at room temperature for fifteen minutes.
- Spin down the sample at 1000 x g for a minute at room temperature to pellet the beads at the bottom of the tube.
- Carefully collect the supernatant, taking care not to disturb the bead pellet. The sample is now ready for analysis.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 10 ml Catalase Agarose Beads 1 x 1ml 1 x 10ml
Images
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Immobilized Catalase beads minimize hydrogen peroxide background in urine adenosine measurement.
Urine adenosine was measured using Abcam’s Adenosine Fluorometric Assay Kit (ab211094) in 2x diluted -untreated and -treated (immobilized catalase beads ab218718 + enzyme mix for ab211094) urine sample. There is a considerable decrease in the sample background in the treated urine as compared to the untreated urine. After background subtraction, linearity across sample volume can be observed in the treated urine sample, but not in the untreated urine sample.
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Immobilized Catalase beads minimize hydrogen peroxide background in urine adenosine measurement.Urine adenosine was measured using Abcam’s Adenosine Fluorometric Assay Kit (ab211094) in 2x diluted -untreated and -treated (immobilized catalase beads ab218718 + enzyme mix for ab211094) urine sample. There is a considerable decrease in the sample background in the treated urine as compared to the untreated urine. In the ab211094 assay, intrinsic hydrogen peroxide and other substrates in the urine sample that also generate hydrogen peroxide are contributing to the background and the immobilized Catalase beads is minimizing such background.
