Anti-Creatine kinase MT (phospho Y153) antibody (ab228517)
Key features and details
- Rabbit polyclonal to Creatine kinase MT (phospho Y153)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Creatine kinase MT (phospho Y153) antibody
See all Creatine kinase MT primary antibodies -
Description
Rabbit polyclonal to Creatine kinase MT (phospho Y153) -
Host species
Rabbit -
Specificity
Specific for endogenous levels of the ~46 kDa Creatine Kinase MT protein phosphorylated at Tyr153. Immunolabeling of Creatine Kinase MT is greatly decreased by lambda phosphatase treatment. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human Creatine kinase MT (phospho Y153).
Database link: P12532 -
Positive control
- WB: Mouse mitochondria from whole brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab228517 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (ab228517)All lanes : Anti-Creatine kinase MT (phospho Y153) antibody (ab228517) at 1/1000 dilution
Lane 1 : Mouse mitochondria from whole brain lysate
Lane 2 : Mouse mitochondria from whole brain lysate treated with lambda phosphatase (1200 units for 30 minutes)
Predicted band size: 47 kDaPhosphospecificity is shown in Lane 2 where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (1200 units for 30 minutes).
