Anti-COX5A antibody [6E9B12D5] (ab110262)
![Anti-COX5A antibody [6E9B12D5] (ab110262)](https://www.abcam.com/ps/products/110/ab110262/Images/ab110262-123453-anti-cox5a-antibody-6e9b12d5-western-blot.gif "Anti-COX5A antibody [6E9B12D5] (ab110262)")
Key features and details
- Mouse monoclonal [6E9B12D5] to COX5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Overview
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Product name
Anti-COX5A antibody [6E9B12D5]
See all COX5A primary antibodies -
Description
Mouse monoclonal [6E9B12D5] to COX5A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Other Immunogen Type. This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
6E9B12D5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)All lanes : Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 17 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)](https://www.abcam.com/ps/products/110/ab110262/Images/ab110262-123452-anti-cox5a-antibody-6e9b12d5-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml). -
![Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)](https://www.abcam.com/ps/products/110/ab110262/Images/ab110262-123451-anti-cox5a-antibody-6e9b12d5-flow-cytometry.jpg)
Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
