Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX5A antibody [EPR14207(B)] (ab181226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14207(B)] to COX5A
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-COX5A antibody [EPR14207(B)]
    See all COX5A primary antibodies

  • Description

    Rabbit monoclonal [EPR14207(B)] to COX5A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa and A431 cell lysates; Human brain and kidney tissues; Hela cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14207(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Western blot - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    All lanes : Anti-COX5A antibody [EPR14207(B)] (ab181226) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] (ab181226)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COX5A with ab181226 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14207(B)] (ab181226)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COX5A with ab181226 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [EPR14207(B)] (ab181226)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling COX5A with ab181226 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Flow Cytometry - Anti-COX5A antibody [EPR14207(B)] (ab181226)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling COX5A with ab181226 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Immunoprecipitation - Anti-COX5A antibody [EPR14207(B)] (ab181226)

    Western blot analysis of HeLa cell lysate immunoprecipitated with ab181226 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-COX5A antibody [EPR14207(B)] (ab181226)
    Anti-COX5A antibody [EPR14207(B)] (ab181226)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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