Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-IL-17F antibody [EPR17830-169] (ab187059)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17830-169] to IL-17F
Suitable for: IP, Flow Cyt, WB
Reacts with: Mouse

Product name

Anti-IL-17F antibody [EPR17830-169]
See all IL-17F primary antibodies
Description

Rabbit monoclonal [EPR17830-169] to IL-17F
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cytometry: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17830-169
Isotype

IgG
Research areas

Flow Cytometry - Anti-IL-17F antibody [EPR17830-169] (ab187059)

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml Ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.

Secondary antibody used Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution.
Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)

All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution

Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 26 seconds

Blocking/Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)

IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.

Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds
Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)

All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution

Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 18 kDa
Observed band size: 16-18 kDa
why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking/Diluting buffer: 5% NFDM/TBST

The molecular mass observed is consistent with the literature (PMID 2212322).

Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).
Anti-IL-17F antibody [EPR17830-169] (ab187059)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com