Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-17F antibody [EPR17830-169] (ab187059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17830-169] to IL-17F
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IL-17F antibody [EPR17830-169]
    See all IL-17F primary antibodies

  • Description

    Rabbit monoclonal [EPR17830-169] to IL-17F

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cytometry: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    Flow Cytometry - Anti-IL-17F antibody [EPR17830-169] (ab187059)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml Ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.

    Secondary antibody used Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

  • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution

    Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
    Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)

    IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
    Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds

  • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
    All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution

    Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 16-18 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST

    The molecular mass observed is consistent with the literature (PMID 2212322).

    Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).

  • Anti-IL-17F antibody [EPR17830-169] (ab187059)
    Anti-IL-17F antibody [EPR17830-169] (ab187059)

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