Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13826(B)] to CoREST - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free
    See all CoREST primary antibodies

  • Description

    Rabbit monoclonal [EPR13826(B)] to CoREST - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250375 is the carrier-free version of ab181139. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250375 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    All lanes : Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181139, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/10000 dilution + MOLT4 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?



    This data was developed using ab181139, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    Flow Cytometry - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    This data was developed using ab181139, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CoREST with ab181139 at 1/150 (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
    Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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