Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
![Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)](https://www.abcam.com/ps/products/250/ab250375/Images/ab250375-405827-anti-corest-antibody-epr13826-b-bsa-and-azide-free-western-blot.jpg "Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13826(B)] to CoREST - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free
See all CoREST primary antibodies -
Description
Rabbit monoclonal [EPR13826(B)] to CoREST - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250375 is the carrier-free version of ab181139. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250375 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13826(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)All lanes : Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?This data was developed using ab181139, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)](https://www.abcam.com/ps/products/250/ab250375/Images/ab250375-402663-anti-corest-antibody-epr13826-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/10000 dilution + MOLT4 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?This data was developed using ab181139, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)](https://www.abcam.com/ps/products/250/ab250375/Images/ab250375-1-anti-corest-antibody-epr13826-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)This data was developed using ab181139, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CoREST with ab181139 at 1/150 (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)](https://www.abcam.com/ps/products/250/ab250375/Images/ab250375-4-benefits-of-recombinant-antibodies.png)
Anti-CoREST antibody [EPR13826(B)] - BSA and Azide free (ab250375)
