Human SUZ12 knockout HeLa cell lysate (ab257721)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260330 - Human SUZ12 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human SUZ12 knockout HeLa cell lysate (ab257721)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: SUZ12 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab175187 observed at 100 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab175187 Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade was shown to specifically react with SUZ12 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264983 (knockout cell lysate ab257721) lane below 100kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175187 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human SUZ12 knockout HeLa cell lysate (ab257721)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: SUZ12 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab12073 Anti-SUZ12 antibody - ChIP Grade was shown to specifically react with SUZ12 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264983 (knockout cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human SUZ12 knockout HeLa cell lysate (ab257721)Homozygous: 23 bp deletion in exon 1
