Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Collagen VI alpha 2 antibody [EPR7889(N)]
See all Collagen VI alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2 -
Host species
Rabbit -
Specificity
This antibody is only recommended for Western blot with mouse samples. Based on our IHC testing data we do not recommend it for IHC with mouse samples. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Collagen VI alpha 2 aa 800-900 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: P12110 -
Positive control
- Human placenta and skin lysates. Human colon and uterus tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7889(N) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 109 kDa
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Immunohistochemical analysis of Human colon tissue, staining Collagen VI alpha 2 with ab180855 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Human uterus tissue, staining Collagen VI alpha 2 with ab180855 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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