Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Collagen III antibody [EPR17673] (ab184993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17673] to Collagen III
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Collagen III antibody [EPR17673]
    See all Collagen III primary antibodies

  • Description

    Rabbit monoclonal [EPR17673] to Collagen III

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, fetal kidney, skin, skeletal muscle and heart lysates; HeLa, MCF7, HaCaT and A431 whole cell lysates. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)
    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)
    All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate at 20 µg
    Lane 2 : Human fetal kidney lysate at 20 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : HaCaT (human keratinocyte cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 139 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 3 seconds; Lanes 4-5: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).

  • Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)
    Western blot - Anti-Collagen III antibody [EPR17673] (ab184993)
    All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : Human skeletal muscle lysate
    Lane 3 : Human heart lysate
    Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 139 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 30 seconds; Lane 4: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (ab184993)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-Collagen III antibody [EPR17673] (ab184993)
    Flow Cytometry - Anti-Collagen III antibody [EPR17673] (ab184993)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Collagen III with ab184993 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)
    Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

    Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab184993 IP in HeLa whole cell lysate . 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Collagen III antibody [EPR17673] (ab184993)
    Anti-Collagen III antibody [EPR17673] (ab184993)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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