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Anti-Collagen III antibody [FH-7A] (ab6310)

Key features and details

  • Mouse monoclonal [FH-7A] to Collagen III
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Rat
  • Isotype: IgG1

Overview

  • Product name

    Anti-Collagen III antibody [FH-7A]
    See all Collagen III primary antibodies

  • Description

    Mouse monoclonal [FH-7A] to Collagen III

  • Host species

    Mouse

  • Specificity

    ab6310 specifically recognizes collagen type III from human and rat origin. It does not recognize collagen types I, II, IV, V, VI and X.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    See all applications and species data

  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control

    • IHC: Human skin sections. WB: recombinant collagen III.

  • General notes

    Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

     

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

    Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310) This image is courtesy of an Abreview submitted by Birgitta Weijdegard

    ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

    This image was generated using the ascites version of the product.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
    Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

    Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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