Anti-Cobra Venom Factor antibody (ab229942)
Key features and details
- Rabbit polyclonal to Cobra Venom Factor
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Cobra Venom Factor antibody -
Description
Rabbit polyclonal to Cobra Venom Factor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Monocled cobra
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Immunogen
Recombinant fragment corresponding to Monocled cobra Cobra Venom Factor aa 700-1000.
Database link: Q91132 -
Positive control
- WB: Cobra Venom Factor recombinant protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Cobra Venom Factor antibody (ab229942)All lanes : Anti-Cobra Venom Factor antibody (ab229942) at 1/500 dilution
Lane 1 : Cobra Venom Factor recombinant protein at 0.08 µg
Lane 2 : Cobra Venom Factor recombinant protein at 0.04 µg
Lane 3 : Cobra Venom Factor recombinant protein at 0.02 µg
Lane 4 : Cobra Venom Factor recombinant protein at 0.01 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 185 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This protein is 185kDa before any post-translational modifications, but it can be split into 3 chains: alpha (aa 23-649), beta (aa 1264-1642) and gamma (aa 733-984). ab229942 detects the gamma chain.