Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
![Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.com/ps/products/109/ab109414/Images/ab109414-122236-anti-bnip3l-antibody-epr4033-western-blot.jpg "Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4033] to BNIP3L/NIX
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BNIP3L/NIX antibody [EPR4033]
See all BNIP3L/NIX primary antibodies -
Description
Rabbit monoclonal [EPR4033] to BNIP3L/NIX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji and K562 cell lysates; Human breast carcinoma tissue
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General notes
This product was previously labelled as BNIP3L
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4033 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)All lanes : Anti-BNIP3L/NIX antibody [EPR4033] (ab109414) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.com/ps/products/109/ab109414/Images/ab109414-122235-anti-bnip3l-antibody-epr4033-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.com/ps/products/109/ab109414/Images/ab109414-3-benefits-of-recombinant-antibodies.png)
Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
