Antibodies, Proteins, Kits and Reagents for Life Science

358 492 ₸ Product size

100 µl 358 492 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4025] to LOX
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-LOX antibody [EPR4025]
See all LOX primary antibodies
Description

Rabbit monoclonal [EPR4025] to LOX
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOX aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P28300
Positive control
- HeLa, Jurkat and WI-38 cell lysates. Human muscle tissue. HeLa cells. Immunoprecipitation pellet from WI-38 cells lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4025
Isotype

IgG
Research areas

Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/3700 dilution (purified)

Lane 1 : WI-38 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse brain
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

Overlay histogram showing Jurkat cells fixed in 2% PFA and stained with purified ab174316 at a dilution of 1 in 90 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1100 dilution (unpurified)

Lane 1 : WI-38 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse brain
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : WI-38 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical analysis of paraffin-embedded human muscle tissue labeling LOX with unpurified ab174316 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab174316 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence staining of Jurkat cells with unpurified ab174316 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence analysis of HeLa cells labeling LOX with unpurified ab174316 at a 1/50 dilution.
Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab174316 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunoprecipitation - Anti-LOX antibody [EPR4025] (ab174316)

Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 (Human fetal lung fibroblast cell line) cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-LOX antibody [EPR4025] (ab174316)

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