Anti-LOX antibody (ab31238)
Key features and details
- Rabbit polyclonal to LOX
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LOX antibody
See all LOX primary antibodies -
Description
Rabbit polyclonal to LOX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Dog
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Immunogen
Synthetic peptide corresponding to Human LOX aa 400 to the C-terminus (C terminal).
(Peptide available asab28612)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human LOX peptide (ab28612). Abcam recommends using Milk as the blocking agent.
Target
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Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. -
Tissue specificity
Heart, placenta, skeletal muscle, kidney, lung and pancreas. -
Involvement in disease
Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. -
Sequence similarities
Belongs to the lysyl oxidase family. -
Post-translational
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 396474 Chicken
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: Q05063 Chicken
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
see all -
Alternative names
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
see all
Images
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Western blot - Anti-LOX antibody (ab31238)Lanes 1-4 : Anti-LOX antibody (ab31238) at 1 µg/ml (Milk blocking)
Lanes 5-8 : Anti-LOX antibody (ab31238) at 1 µg/ml (BSA blocking)
Lanes 1 & 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 3 & 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 4 & 8 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk (lanes 1-4) or 2% Bovine Serum Albumin (lanes 5-8) before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-LOX antibody (ab31238)Anti-LOX antibody (ab31238) at 1 µg/ml + MDA-MB-361 whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Western blot - Anti-LOX antibody (ab31238)All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Additional bands at: 15 kDa, 36 kDa (possible mature (processed) protein), 47 kDa (possible immature (unprocessed)), 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody (ab31238)Image from Brown JP et al., PLoS One. 2012;7(6):e38475. Epub 2012 Jun 7. Fig 7.; doi:10.1371/journal.pone.0038475; June 7, 2012, PLoS ONE 7(6): e38475.Immunohistochemical analysis of murine embryonic spinal tissue, staining LOX with ab31238.
Tissue was permeabilized with 0.025% Triton-X and blocked for nonspecific binding using blocking solution. Sections were incubated overnight with primary antibody (1/100) and staining was detected using DAB.
References (54)
ab31238 has been referenced in 54 publications.
- Hirakata S et al. Genetic Deletion of Socs3 in Smooth Muscle Cells Ameliorates Aortic Dissection in Mice. JACC Basic Transl Sci 5:126-144 (2020). PubMed: 32140621
- Kalikawe R et al. Lysyl oxidase impacts disease outcomes and correlates with global DNA hypomethylation in esophageal cancer. Cancer Sci 110:3727-3737 (2019). PubMed: 31599475
- Daley EJ et al. Impaired Gastric Hormone Regulation of Osteoblasts and Lysyl Oxidase Drives Bone Disease in Diabetes Mellitus. JBMR Plus 3:e10212 (2019). PubMed: 31687648
- Yang M et al. Lysyl oxidase assists tumor-initiating cells to enhance angiogenesis in hepatocellular carcinoma. Int J Oncol 54:1398-1408 (2019). PubMed: 30720077
- Bai L et al. Bone morphogenetic protein 2 increases lysyl oxidase activity via up-regulation of snail in human granulosa-lutein cells. Cell Signal 53:201-211 (2019). PubMed: 30321593
Images
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Western blot - Anti-LOX antibody (ab31238)Lanes 1-4 : Anti-LOX antibody (ab31238) at 1 µg/ml (Milk blocking)
Lanes 5-8 : Anti-LOX antibody (ab31238) at 1 µg/ml (BSA blocking)
Lanes 1 & 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 3 & 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lanes 4 & 8 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk (lanes 1-4) or 2% Bovine Serum Albumin (lanes 5-8) before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Western blot - Anti-LOX antibody (ab31238)Anti-LOX antibody (ab31238) at 1 µg/ml + MDA-MB-361 whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
-
Western blot - Anti-LOX antibody (ab31238)All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Additional bands at: 15 kDa, 36 kDa (possible mature (processed) protein), 47 kDa (possible immature (unprocessed)), 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody (ab31238) Image from Brown JP et al., PLoS One. 2012;7(6):e38475. Epub 2012 Jun 7. Fig 7.; doi:10.1371/journal.pone.0038475; June 7, 2012, PLoS ONE 7(6): e38475.Immunohistochemical analysis of murine embryonic spinal tissue, staining LOX with ab31238.
Tissue was permeabilized with 0.025% Triton-X and blocked for nonspecific binding using blocking solution. Sections were incubated overnight with primary antibody (1/100) and staining was detected using DAB.
