Anti-Coatomer subunit delta/COPD antibody (ab96725)
Key features and details
- Rabbit polyclonal to Coatomer subunit delta/COPD
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Coatomer subunit delta/COPD antibody -
Description
Rabbit polyclonal to Coatomer subunit delta/COPD -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment corresponding to Human Coatomer subunit delta/COPD aa 89-492.
Database link: NP_001646 -
Positive control
- WB: 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates. ICC/IF: HeLa cells. IHC-P: Mouse brain tissue. IHC-Fr: Mouse cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Coatomer subunit delta/COPD antibody (ab96725)
Frozen section of mouse cerebellum tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/250 dilution (green). The nuclear counter stain is DAPI (blue). NF-H is detected with an anti NF-H antibody (red).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coatomer subunit delta/COPD antibody (ab96725)Paraffin-embedded mouse brain tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
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Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (ab96725)Paraformaldehyde-fixed HeLa cells stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-tubulin antibody (red).
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Western blot - Anti-Coatomer subunit delta/COPD antibody (ab96725)All lanes : Anti-Coatomer subunit delta/COPD antibody (ab96725) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (ab96725)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.