Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CMTM6 antibody [EPR23015-45] (ab264067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23015-45] to CMTM6
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CMTM6 antibody [EPR23015-45]
    See all CMTM6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23015-45] to CMTM6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231 and BxPC-3 whole cell lysates; human tonsil tissue lysate. IHC-P: Human tonsil, thyroid cancer and pancreatic cancer tissue. Flow Cyt: MDA-MB-231 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23015-45

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    All lanes : Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Human tonsil tissue lysate
    Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-2: 3 minutes

    Lane 3: 48 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28813417).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID: 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Flow Cytometry - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-CMTM6 antibody [EPR23015-45] (ab264067)
    Anti-CMTM6 antibody [EPR23015-45] (ab264067)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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