Anti-CHRNA6 antibody [5B6G8] (ab233721)
![Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307144-anti-chrna6-antibody-5b6g8-western-blot.jpg "Anti-CHRNA6 antibody [5B6G8] (ab233721)")
Key features and details
- Mouse monoclonal [5B6G8] to CHRNA6
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CHRNA6 antibody [5B6G8]
See all CHRNA6 primary antibodies -
Description
Mouse monoclonal [5B6G8] to CHRNA6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CHRNA6 aa 26-239. (Expressed in E.coli).
Database link: Q15825 -
Positive control
- WB: Recombinant human CHRNA6 (aa 26-239) protein; CHRNA6 (aa 26-239)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human bladder cancer tissue. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y and SK-N-SH cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5B6G8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)Anti-CHRNA6 antibody [5B6G8] (ab233721) + Recombinant human CHRNA6 (aa 26-239) protein
Predicted band size: 57 kDaExpected MW is 51.2 kDa.
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![Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307147-anti-chrna6-antibody-5b6g8-western-blot.jpg)
Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)All lanes : Anti-CHRNA6 antibody [5B6G8] (ab233721) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CHRNA6 (aa 26-239)-hIgGFc transfected HEK HEK-293 cell lysate
Predicted band size: 57 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307154-anti-chrna6-antibody-5b6g8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRNA6 (green) using ab233721 at 1/100 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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![Flow Cytometry - Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307155-anti-chrna6-antibody-5b6g8-flow-cytometry.jpg)
Flow Cytometry - Anti-CHRNA6 antibody [5B6G8] (ab233721)Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
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![Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307159-anti-chrna6-antibody-5b6g8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA6 antibody [5B6G8] (ab233721)](https://www.abcam.com/ps/products/233/ab233721/Images/ab233721-307160-anti-chrna6-antibody-5b6g8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA6 antibody [5B6G8] (ab233721)Paraffin-embedded human bladder cancer tissue stained for CHRNA6 using ab233721 at 1/200 dilution in immunohistochemical analysis. DAB staining.
