Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHRNA6 antibody [5B6G8] (ab233721)

Key features and details

  • Mouse monoclonal [5B6G8] to CHRNA6
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CHRNA6 antibody [5B6G8]
    See all CHRNA6 primary antibodies

  • Description

    Mouse monoclonal [5B6G8] to CHRNA6

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CHRNA6 aa 26-239. (Expressed in E.coli).
    Database link: Q15825

  • Positive control

    • WB: Recombinant human CHRNA6 (aa 26-239) protein; CHRNA6 (aa 26-239)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human bladder cancer tissue. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y and SK-N-SH cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    5B6G8

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Anti-CHRNA6 antibody [5B6G8] (ab233721) + Recombinant human CHRNA6 (aa 26-239) protein

    Predicted band size: 57 kDa



    Expected MW is 51.2 kDa.

  • Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Western blot - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    All lanes : Anti-CHRNA6 antibody [5B6G8] (ab233721) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : CHRNA6 (aa 26-239)-hIgGFc transfected HEK HEK-293 cell lysate

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRNA6 (green) using ab233721 at 1/100 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow Cytometry - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Flow Cytometry - Anti-CHRNA6 antibody [5B6G8] (ab233721)

    Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Immunocytochemistry/ Immunofluorescence - Anti-CHRNA6 antibody [5B6G8] (ab233721)

    Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA6 antibody [5B6G8] (ab233721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA6 antibody [5B6G8] (ab233721)

    Paraffin-embedded human bladder cancer tissue stained for CHRNA6 using ab233721 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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