Anti-CEBP Beta antibody (ab264305)
Key features and details
- Rabbit polyclonal to CEBP Beta
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEBP Beta antibody
See all CEBP Beta primary antibodies -
Description
Rabbit polyclonal to CEBP Beta -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide within Human CEBP Beta aa 50-100. The exact sequence is proprietary.
Database link: P17676 -
Positive control
- IHC-P: Human prostate carcinoma. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CEBP Beta antibody (ab264305)All lanes : Anti-CEBP Beta antibody (ab264305) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : Jurkat whole cell lysate at 50 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
4-20% SDS-PAGE.
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Immunoprecipitation - Anti-CEBP Beta antibody (ab264305)CEBP Beta was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264305 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264305 at 1 µg/ml.
Lane 1: ab264305 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 mins.
Cells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Beta antibody (ab264305)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CEBP Beta with ab264305 at 1/1,000 dilution. Detection: DAB.
