Anti-CEACAM20 antibody [HT-12D8] (ab59626)
Key features and details
- Mouse monoclonal [HT-12D8] to CEACAM20
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-CEACAM20 antibody [HT-12D8] -
Description
Mouse monoclonal [HT-12D8] to CEACAM20 -
Host species
Mouse -
Specificity
ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human, Chinese hamster -
Immunogen
Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20
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Positive control
- CHO cells. FACS: BOSC23 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab59626 was purified by protein G affinity chromatography from cell culture supernatants. -
Clonality
Monoclonal -
Clone number
HT-12D8 -
Isotype
IgG1 -
Research areas
Images
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Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).