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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CEACAM20 antibody [HT-12D8] (ab59626)

Anti-CEACAM20 antibody [HT-12D8] (ab59626)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [HT-12D8] to CEACAM20
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human, Chinese hamster
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CEACAM20 antibody [HT-12D8]
  • Description

    Mouse monoclonal [HT-12D8] to CEACAM20
  • Host species

    Mouse
  • Specificity

    ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Chinese hamster
  • Immunogen

    Other Immunogen Type corresponding to Human CEACAM20. cDNA encoding human CEACAM20

  • Positive control

    • CHO cells. FACS: BOSC23 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab59626 was purified by protein G affinity chromatography from cell culture supernatants.
  • Clonality

    Monoclonal
  • Clone number

    HT-12D8
  • Isotype

    IgG1
  • Research areas

    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
    Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
    Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
  • Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
    Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (ab59626)
    BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: a-myc; CEACAM20: a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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