Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19878] to CEACAM1+CEACAM6 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free
    See all CEACAM1+CEACAM6 primary antibodies

  • Description

    Rabbit monoclonal [EPR19878] to CEACAM1+CEACAM6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251459 is the carrier-free version of ab206431. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19878

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : Human CEACAM1 recombinant protein
    Lane 2 : Human CEACAM5 recombinant protein
    Lane 3 : Human CEACAM6 recombinant protein
    Lane 4 : Human CEACAM7 recombinant protein
    Lane 5 : Human CEACAM8 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa


    Exposure time: 1 second


    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human CEACAM1 recombinant protein contains aa35-428 with GST-tag and His-Tag®. Human CEACAM5, CEACAM6, CEACAM7, CEACAM8 recombinant proteins contain aa35-422, aa35-326, aa35-326 and aa35-335 respectively, and all with a His-Tag®.

  • Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 110-150,75-90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, 10751340, 17081782).

  • Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa
    Observed band size: 110-150 kDa why is the actual band size different from the predicted?



    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 minute; Lane 2: 3 seconds; Lane 3: 30 seconds; Lane 4: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123; 10751340; 17081782).

  • Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Western blot - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa


    Exposure time: 3 minutes


    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    HUVEC does not express CEACAM 6 and PC-3 does not express CEACAM 1 (PMID: 22918079, 10359532).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    This data was developed using ab206431, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Brush border staining on human colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    This data was developed using ab206431, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human liver cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)
    Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free (ab251459)

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