Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [11-1H] to CEACAM1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Rat

Overview

  • Product name

    Anti-CEACAM1 antibody [11-1H] - BSA and Azide free
    See all CEACAM1 primary antibodies

  • Description

    Mouse monoclonal [11-1H] to CEACAM1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Recombinant fragment within Rat CEACAM1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P16573

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat liver lysate. IHC-P: Rat liver tissue. IHC-Fr: Rat liver tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255909 is the carrier-free version of ab254156. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    11-1H

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)
    Immunohistochemistry (Frozen sections) - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling CEACAM1 with ab254156 at 5.62 ug/mL followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat liver is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254156).

  • Western blot - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)
    Western blot - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)
    Anti-CEACAM1 antibody [11-1H] (ab254156) at 0.562 µg/ml + Rat liver tissue lysate at 10 µg

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L), at 1/10000 dilution

    Predicted band size: 57 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254156).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CEACAM1 with ab254156 at 2.81 ug/mL followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat liver. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254156).

  • Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)
    Anti-CEACAM1 antibody [11-1H] - BSA and Azide free (ab255909)

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