Anti-CDK1 (phospho Y15) antibody (ab47594)
Key features and details
- Rabbit polyclonal to CDK1 (phospho Y15)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CDK1 (phospho Y15) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho Y15) -
Host species
Rabbit -
Specificity
This antibody is specific for CDK1. -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanAfrican green monkey
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Immunogen
Synthetic peptide corresponding to Human CDK1 aa 5-54.
Database link: P06493
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS-7 cells (African green monkey kidney fibroblast-like)
Predicted band size: 34 kDa
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594) This image is courtesy of an anonymous abreview.All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution
Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : polyclonal goat HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteBlocking: 5% milk for 1 hour at 25°C.
