Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CDCA5 antibody [EPR16331] - C-terminal
    See all CDCA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, HeLa, Jurkat and SH-SY5Y cell lysates; Human stomach and human endometrium adenocarcinoma tissues; HeLa and 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 28 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

     

  • Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

    Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

    Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 28 kDa

  • Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
    Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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