Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
![Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230013-anti-cdca5-antibody-epr16331-c-terminal-western-blot.jpg "Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CDCA5 antibody [EPR16331] - C-terminal
See all CDCA5 primary antibodies -
Description
Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, Jurkat and SH-SY5Y cell lysates; Human stomach and human endometrium adenocarcinoma tissues; HeLa and 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16331 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-253279-anti-cdca5-antibody-epr16331-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230017-anti-cdca5-antibody-epr16331-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230015-anti-cdca5-antibody-epr16331-c-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230014-anti-cdca5-antibody-epr16331-c-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230012-anti-cdca5-antibody-epr16331-c-terminal-western-blot.jpg)
Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa
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![Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-8-benefits-of-recombinant-antibodies.png)
Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
