Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL66] to CD8 alpha - BSA and Azide free
  • Suitable for: ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CD8 alpha antibody [CAL66] - BSA and Azide free
    See all CD8 alpha primary antibodies

  • Description

    Rabbit monoclonal [CAL66] to CD8 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human CD8 alpha aa 204-235. The exact sequence is proprietary.
    Database link: P01732

  • Positive control

    • IHC-P: Human tonsil, colon and gastric carcinoma tissue; rat spleen tissue. ICC/IF: Human PBMCs. Flow cyt: Human PBMCs. IP: Human thymus lysate.

  • General notes

    Ab251596 is the carrier-free version of ab237709. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251596 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes in human colon is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling CD8 alpha with ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes in human gastric carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD8 alpha with ab237709 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the rat spleen is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (peripheral blood mononuclear cells) labeling CD8 alpha with ab237709 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC. The nuclear counter stain is DAPI (blue). CD3 is detected with an anti-CD3 antibody-Alexa Fluor® 647 comjugate at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    CD8 alpha was immunoprecipitated from 0.35 mg of human thymus lysate with ab237709 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237709 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Human thymus lysate 10 μg (Input). 
    Lane 2: ab237709 IP in human thymus lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237709 in human thymus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 100 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeacblized human PBMC (peripheral blood mononuclear cells) labeling CD8 alpha with ab237709 at 1/500 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). 

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD8 alpha conjugated to BV421.The fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or ab237709 (Right). They recognize same populations.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab237709 at 0.25 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237709).

  • Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
    Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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