Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)

Key features and details

  • Mouse monoclonal [2D1B3] to CD74 - N-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD74 antibody [2D1B3] - N-terminal
    See all CD74 primary antibodies

  • Description

    Mouse monoclonal [2D1B3] to CD74 - N-terminal

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human CD74 aa 1-106 (N terminal). Expressed in E.coli.
    Sequence:

    MHRRRSRSCR EDQKPVMDDQ RDLISNNEQL PMLGRRPGAP ESKCSRGALY TGFSILVTLL LAGQATTAYF LYQQQGRLDK LTVTSQNLQL ENLRMKLPKP PKPVSK


    Database link: P04233
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • CD74 recombinant protein, CD74 (amino acids: 1-106)-hIgGFc transfected HEK293 cell lysate, Raji cell lysate, HeLa cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    2D1B3

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Raji cell line lysate with Anti-CD74 antibody [2D1B3] - N-terminal (ab181465) at 1/500 dilution

    Predicted band size: 34 kDa
  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)

    Immunofluorescence analysis of HeLa cells labeling CD74 using ab181465 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Anti-CD74 antibody [2D1B3] - N-terminal (ab181465) at 1/500 dilution + Human CD74 recombinant protein

    Predicted band size: 34 kDa

  • Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    Western blot - Anti-CD74 antibody [2D1B3] - N-terminal (ab181465)
    All lanes : Anti-CD74 antibody [2D1B3] - N-terminal (ab181465) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : CD74 (amino acids: 1-106)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 34 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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