Anti-CD6/T12 antibody [EPR4056] (ab109218)
![Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121863-anti-cd6-t12-antibody-epr4056-western-blot.jpg "Anti-CD6/T12 antibody [EPR4056] (ab109218)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4056] to CD6/T12
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD6/T12 antibody [EPR4056]
See all CD6/T12 primary antibodies -
Description
Rabbit monoclonal [EPR4056] to CD6/T12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HuT-78, PBMC and Human tonsil lysates IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as CD6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4056 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD6/T12 antibody [EPR4056] (ab109218)All lanes : Anti-CD6/T12 antibody [EPR4056] (ab109218) at 1/1000 dilution
Lane 1 : Jurkat lysates
Lane 2 : HuT-78 lysates
Lane 3 : PBMC lysates
Lane 4 : Human tonsil lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121862-anti-cd6-t12-antibody-epr4056-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)ab109218, at a 1/100 dilution, staining CD6/T12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121861-anti-cd6-t12-antibody-epr4056-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)ab109218, at a 1/100 dilution, staining CD6/T12 in paraffin embedded Human lymphocytes infiltrating colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121860-anti-cd6-t12-antibody-epr4056-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)ab109218 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121859-anti-cd6-t12-antibody-epr4056-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)ab109218 showing negative staining in Normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-121858-anti-cd6-t12-antibody-epr4056-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (ab109218)ab109218 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CD6/T12 antibody [EPR4056] (ab109218)](https://www.abcam.com/ps/products/109/ab109218/Images/ab109218-7-benefits-of-recombinant-antibodies.png)
Anti-CD6/T12 antibody [EPR4056] (ab109218)
