Anti-CD63 antibody [MEM-259] (ab8219)
Key features and details
- Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies -
Description
Mouse monoclonal [MEM-259] to CD63 -
Host species
Mouse -
Specificity
CD63 antigen (human) -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Tissue/ cell preparation (Human). (HPB-ALL T cell line)
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Positive control
- IHC: Human spleen sections; ICC: Human HeLa cells, primary fibroblasts and skin fibroblasts tissues; Flow cyt: Human blood samples.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-259 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (ab8219)Immunohistochemistry staining of human spleen (paraffin sections) using ab8219.
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Immunocytochemistry staining of human skin fibroblasts with ab8219 (green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
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Flow Cytometry - Anti-CD63 antibody [MEM-259] (ab8219) This image is courtesy of an anonymous Abreviewab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody. -
Immunocytochemistry staining of CD63 in human HeLa cell line using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml).
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Immunocytochemistry staining of CD63 in human primary fibroblasts using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).