Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [KILL150A] to CD63 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD63 antibody [KILL150A] - BSA and Azide free
    See all CD63 primary antibodies

  • Description

    Mouse monoclonal [KILL150A] to CD63 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-MEL-28 and HUVEC whole cell lysate. Human lymph node tissue lysate. IHC-P: Human melanoma tissue. ICC/IF: SK-MEL-28 cells. Flow Cyt: SK-MEL-28 and NK-92 cells.

  • General notes

    ab271296 is the carrier-free version of ab271286. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271296 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Western blot - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    All lanes : Anti-CD63 antibody [KILL150A] (ab271286) at 0.978 µg/ml

    Lane 1 : SK-MEL-28 (human malignant melanoma), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 3 : Human lymph node tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 26 kDa



    CD63 can undergo glycosylation as shown in lane 1, 2 and 3 (PMID: 9890706, 28740179).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 mins; Lane 3: 48 secs.

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human melanoma. The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: Nearly no staining on human breast.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling CD63 with ab271286 at 1/50 dilution (19.56µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing lysosome/endosomes (ab252919) co-staining in SK-MEL-28 cells.

    ab252919 Anti-CD63 rabbit monoclonal antibody was used to counterstain tubulin at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab271286 at a 1/50 dilution (19.56µg/ml) followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab259919 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Flow Cytometry - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Flow Cytometry - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

    Flow cytometric analysis of NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labelling CD63 with ab271286 at 1/1000 dilution (19.56µg/ml) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Flow Cytometry - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Flow Cytometry - Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

    Flow cytometric analysis of SK-MEL-28 (human malignant melanoma) cells labelling CD63 with ab271286 at 1/1000 dilution (19.56µg/ml) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271286).

  • Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)
    Anti-CD63 antibody [KILL150A] - BSA and Azide free (ab271296)

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