Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD55 antibody [EPR22362-255] (ab243231)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22362-255] to CD55
Suitable for: ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CD55 antibody [EPR22362-255]
See all CD55 primary antibodies
Description

Rabbit monoclonal [EPR22362-255] to CD55
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

ICC/IF
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: Human peripheral blood mononuclear cells. Flow cyt: Human peripheral blood mononuclear cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
ICC/IF	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22362-255
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD55 antibody [EPR22362-255] (ab243231)

Flow cytometric analysis of IMR-32 (human neuroblastoma neuroblast cell line) cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ^® 488), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control: IMR-32 (PMID: 27043658 and 15699172).
Flow Cytometry - Anti-CD55 antibody [EPR22362-255] (ab243231)

Flow cytometric analysis of human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ^® 488), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Relative levels of CD55 expression on monocytes (SSC high population) and lymphocyte (main population, SSC low) subsets was described in the literature (PMID: 16999828).
Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [EPR22362-255] (ab243231)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution.

Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: IMR-32 (PMID: 27043658).
Anti-CD55 antibody [EPR22362-255] (ab243231)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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