Allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate.

Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, membrane structural components, and energy reservoirs. Simple phosphatases, such as alkaline and acid phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Alkaline phosphatase plays an important role in many biochemical regulatory pathways, including a possible role in cell differentiation. Alkaline phosphatase activity can be monitored using the fluorogenic substrate, Fluorescein diphosphate, tetraammonium salt (FDP) contained in this kit. Alkaline phosphatase catalyzes a two-step hydrolysis of FDP, releasing the highly fluorescent compound, fluorescein, and activity measurements are easily obtained either in vitro, in cell preparations, or in vivo. The kit contains enough substrate for numerous assays and control experiments, and also contains reference standards and a detailed protocol for use. See the references below for more information and applications.

This kit is sold by Marker Gene under product code M1222.