Anti-CD44 antibody [M591] (ab253279)
![Anti-CD44 antibody [M591] (ab253279)](https://www.abcam.com/ps/products/253/ab253279/Images/ab253279-344294-AntiCD44-antibody-M591-immunocytochemistry-mcf7-human.jpg "Anti-CD44 antibody [M591] (ab253279)")
Key features and details
- Mouse monoclonal [M591] to CD44
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD44 antibody [M591]
See all CD44 primary antibodies -
Description
Mouse monoclonal [M591] to CD44 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type within CD44. The exact sequence is proprietary. Corresponding to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line.
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Positive control
- WB: MDA-MB-231 whole cell lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M591 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M591] (ab253279)
Paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling CD44 using ab253279 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD44 antibody [M591] (ab253279)](https://www.abcam.com/ps/products/253/ab253279/Images/ab253279-344293-AntiCD44-antibody-M591-western-blot-mdamb231-human.jpg)
Western blot - Anti-CD44 antibody [M591] (ab253279)All lanes : Anti-CD44 antibody [M591] (ab253279) at 1/1000 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, denatured conditions
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, native conditions
Predicted band size: 81 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.