Anti-CD44 antibody [M024] - Extracellular domain (ab253277)
![Anti-CD44 antibody [M024] - Extracellular domain (ab253277)](https://www.abcam.com/ps/products/253/ab253277/Images/ab253277-344290-AntiCD44-antibody-M024--Extracellular-domain-immunocytochemistry-mewo-human.jpg "Anti-CD44 antibody [M024] - Extracellular domain (ab253277)")
Key features and details
- Mouse monoclonal [M024] to CD44 - Extracellular domain
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-CD44 antibody [M024] - Extracellular domain
See all CD44 primary antibodies -
Description
Mouse monoclonal [M024] to CD44 - Extracellular domain -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Other Immunogen Type within Human CD44 (extracellular). The exact sequence is proprietary. MeWo melanoma cell line.
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Positive control
- ICC/IF: MeWo cells. WB: A549, MDA-MB-231, MeWo cell lysate. Human breast, lung, skin and brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M024 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M024] - Extracellular domain (ab253277)
Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling CD44 using ab253277 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD44 antibody [M024] - Extracellular domain (ab253277)](https://www.abcam.com/ps/products/253/ab253277/Images/ab253277-344289-AntiCD44-antibody-M024--Extracellular-domain-western-blot-a549-mewo-mdamb231-breast-skin-lung-brain-human.jpg)
Western blot - Anti-CD44 antibody [M024] - Extracellular domain (ab253277)All lanes : Anti-CD44 antibody [M024] - Extracellular domain (ab253277) at 1/2000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : MeWo (Human malignant melanoma cell line) cell lysate
Lane 4 : Human breast tissue lysate
Lane 5 : Human lung tissue lysate
Lane 6 : Human skin tissue lysate
Lane 7 : Human brain tissue lysate
Predicted band size: 81 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.
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![ELISA - Anti-CD44 antibody [M024] - Extracellular domain (ab253277)](https://www.abcam.com/ps/products/253/ab253277/Images/ab253277-382886-anti-cd44-antibody-m024-extracellular-domain-elisa.jpg)
ELISA - Anti-CD44 antibody [M024] - Extracellular domain (ab253277)Representative Standard Curve using mouse monoclonal anti-CD44 for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
