All lanes : Anti-GJB2 antibody (ab59020) at 1 µg/ml

Lane 1 : Human colon lysate in RIPA buffer
Lane 2 : Human heart lysate in RIPA buffer
Lane 3 : Human prostate lysate in RIPA buffer
Lane 4 : Human skin lysate in RIPA buffer
Lane 5 : Human adipose lysate in RIPA buffer

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 26 kDa
Observed band size: 25-26 kDa why is the actual band size different from the predicted?

Primary incubation: 1 hour at room temperature.

Lane 1 : Anti-GJB2 antibody (ab59020) at 0.01 µg/ml
Lanes 2-3 : Anti-GJB2 antibody (ab59020) at 0.5 µg/ml

Lane 1 : Mouse brain lysate in RIPA buffer
Lane 2 : Mouse heart lysate in RIPA buffer
Lane 3 : Rat heart lysate in RIPA buffer

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 26 kDa
Observed band size: 25-26 kDa why is the actual band size different from the predicted?

Primary incubation: 1 hour at room temperature.

All lanes : Anti-GJB2 antibody (ab59020) at 1/5000 dilution

Lane 1 : MCF-7 cells
Lane 2 : HCC1806 cells

Secondary
All lanes : HRP-conjugated rabbit anti-goat IgG polyclonal at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 26 kDa
Observed band size: 26 kDa


Exposure time: 5 seconds

Blocked with 5% milk for 1 hour at 25°C.

See Abreview