Anti-CD44 antibody [DF1485] (ab270255)
Key features and details
- Mouse monoclonal [DF1485] to CD44
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD44 antibody [DF1485]
See all CD44 primary antibodies -
Description
Mouse monoclonal [DF1485] to CD44 -
Host species
Mouse -
Specificity
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Human CD44. Purified CD44 antigen (PGp-1) from lymphocyte membrane
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Positive control
- IHC-P: Human breast tissue. ICC/IF:HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate -
Clonality
Monoclonal -
Clone number
DF1485 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Confocal immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD44 using ab270255 at 2 μg/ml followed by a CF488 secondary antibody (Green). Nuclei are stained using Reddot (Red).
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Formalin-fixed, paraffin-embedded human breast tissue stained for CD44 using ab270255 at 2 μg/ml in imunnohistochemical analysis.