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Anti-CD44 antibody [DF1485] (ab270255)

Anti-CD44 antibody [DF1485] (ab270255)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [DF1485] to CD44
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD44 antibody [DF1485]
    See all CD44 primary antibodies
  • Description

    Mouse monoclonal [DF1485] to CD44
  • Host species

    Mouse
  • Specificity

    Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin.

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Human CD44. Purified CD44 antigen (PGp-1) from lymphocyte membrane

  • Positive control

    • IHC-P: Human breast tissue. ICC/IF:HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate
  • Clonality

    Monoclonal
  • Clone number

    DF1485
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Tumor biomarkers
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Images

  • Immunofluorescence - Anti-CD44 antibody [DF1485] (ab270255)
    Immunofluorescence - Anti-CD44 antibody [DF1485] (ab270255)

    Confocal immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD44 using ab270255 at 2 μg/ml  followed by a CF488 secondary antibody (Green). Nuclei are stained using Reddot (Red).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [DF1485] (ab270255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [DF1485] (ab270255)

    Formalin-fixed, paraffin-embedded human breast tissue stained for CD44 using ab270255 at 2 μg/ml in imunnohistochemical analysis.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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