C57BL/6 mouse splenocytes stained with ab61400 (right) or Rat IgG2aΚ (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab61400) or Rat IgG2a Isotype (1x10⁶ in 100µl at 1 µg/ml) (left) for 30 min on ice.

The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°. The cells were simultaneously stained with CD19 APC conjugated antibody.

Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

IHC image of CD38 staining in a section of frozen normal mouse Spleen.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab61400 at 1µg/ml. The section was then incubated with ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) preabsorbed, (Shown in green) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.