Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit recombinant multiclonal [RM1006] to CD31
Suitable for: IP, IHC-Fr, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-CD31 antibody [RM1006]
See all CD31 primary antibodies
Description

Rabbit recombinant multiclonal [RM1006] to CD31
Host species

Rabbit
Tested applications

Suitable for: IP, IHC-Fr, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC, bEnd.3 lysates. IHC-P: Human stomach, Mouse stomach, Rat stomach tissues. IHC-Fr: Mouse lung, Rat lung tissues. IP: THP-1, bEnd.3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Recombinant Multiclonal
Clone number

RM1006
Isotype

IgG

Western blot - Anti-CD31 antibody [RM1006] (ab281583)

All lanes : Anti-CD31 antibody [RM1006] (ab281583) at 1/1000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 2 : bEnd.3 (Mouse brain endothelioma ) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Rat lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 82 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration:5% NFDM/TBST

Negative control: NIH/3T3 (PMID: 1429859).

Exposure time: 37 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (ab281583)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling CD31 with 281583 at 1/5000 (0.092 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human stomach (PMID: 23090636), The section was incubated with ab281583 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-CD31 antibody [RM1006] (ab281583)

CD31 was immunoprecipitated from 0.35 mg THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10 ug with 281583 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 281583 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10 ug

Lane 2: ab281583 IP in THP-1 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281583 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 6 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (ab281583)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling CD31 with 281583 at 1/5000 (0.092 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of mouse stomach, The section was incubated with ab281583 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [RM1006] (ab281583)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling CD31 with 281583 at 1/50 (9.24 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (ab281583)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labelling CD31 with 281583 at 1/5000 (0.092 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of rat stomach. The section was incubated with ab281583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [RM1006] (ab281583)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling CD31 with 281583 at 1/50 (9.24 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-CD31 antibody [RM1006] (ab281583)

CD31 was immunoprecipitated from 0.35 mg bEnd.3 (Mouse brain endothelioma ) whole cell lysate 10 ug with 281583 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 281583 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: bEnd.3 (Mouse brain endothelioma ) whole cell lysate 10 ug

Lane 2: ab281583 IP in bEnd.3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281583 in bEnd.3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 6 seconds.
Anti-CD31 antibody [RM1006] (ab281583)

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