Mitochondria/Cytosol Fractionation Kit (ab65320)
Key features and details
- Assay type: Direct
- Assay time: 4 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Mitochondria/Cytosol Fractionation Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
4h 00m -
Product overview
Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
Visit our FAQs page for tips and troubleshooting. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 25 tests 5X Cytosol Extraction Buffer 1 x 20ml 1 x 5ml DTT 1 x 110µl 1 x 110µl Mitochondria Extraction Buffer 1 x 10ml 1 x 2.5ml Protease Inhibitor Cocktail 1 vial 1 vial -
Research areas
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Relevance
Mitochondria/Cytosol Fractionation is the process for effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of mammalian cells.
