Anti-CD20 antibody [GOT214A] (ab271288)
![Anti-CD20 antibody [GOT214A] (ab271288)](https://www.abcam.com/ps/products/271/ab271288/Images/ab271288-2-anti-cd20-antibody-got214a-immunocytochemistry-mouse-pbmc.jpg "Anti-CD20 antibody [GOT214A] (ab271288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [GOT214A] to CD20
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-CD20 antibody [GOT214A]
See all CD20 primary antibodies -
Description
Rat monoclonal [GOT214A] to CD20 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P Mouse
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen tissue. ICC: Mouse PBMC cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
GOT214A -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] (ab271288)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling CD20 with ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse PBMC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution(Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Used ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150080 at 1/500 dilution.
-ve control 2: Used ab179513 at 1/200 dilution, followed by ab150157 at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] (ab271288)](https://www.abcam.com/ps/products/271/ab271288/Images/ab271288-1-anti-cd20-antibody-got214a-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] (ab271288)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD20 with ab271288 at 1/100 (8.02 μg/mL) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Membranous staining on the mouse spleen is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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![Anti-CD20 antibody [GOT214A] (ab271288)](https://www.abcam.com/ps/products/271/ab271288/Images/ab271288-3-benefits-of-recombinant-antibodies.png)
Anti-CD20 antibody [GOT214A] (ab271288)
